Journals Proceedings

Abstract

In view of medicinal utilities of neem plant have been described especially for in ayurvedic remedy for alleviating of liver disorders, the present study was designed to evaluate the evidence of acute toxicity of lyophilized neem leaf powder (LNLP) and its preventive effects against carbon tetrachloride (CCl4) induced hepatotoxicity in rats. Group allocation in hepatoprotective activity study included vehicle, CCl4 (1ml/kg), Silymarin (100µg/kg/day) + CCl4 and graded doses of LNLP (50 and 100mg/ kg, BW/ day) + CCl4. On 9th day, blood was obtained for determination of biochemical parameters and liver tissue for pathological examination. There were no toxicological effects as evidenced by indications of mortality, behavior, diet consumption and tissue weights, though, some hematological parameters showed variations in their value at higher dose level. The level of protection was deliberated by using various biochemical parameters like total protein, albumin, BUN, AST, ALT and ALP levels. LNLP showed dose dependent hepatoprotective in nature which was further validated by marked diminish in occurrence of hepatocellular necrosis on histopathological analysis. This study suggests LNLP possess hepatoprotective effect against CCl4 induced liver damage in rats with efficiency similar to standard drug and possible mechanism behind the results is discussed.