Journals Proceedings

International Journal of Applied Science and Environmental Engineering

A Novel Immunosorbent Assay for the Detection of Brominated Flanme Retardant-- Tetrabromobisphenol A

Author(s) : BU DAN, ZHUANG HUI SHENG

Abstract

Tetrabromobisphenol A (TBBPA) is a kind of brominated flame retardants widely used in the world. A sensitive and selective competitive indirect biotin-stretavidin amplified emzyme-linked immunosorbent assay (BA-ELISA) for the detection of TBBPA was developed. The limit of detection (IC10) and the inhibition half-maximum concentration (IC50) were 0.027ng/mL and 0.58ng/mL, respectively. Cross-reactivity values of the BA-ELISA with a set of analogues revealed a high selective. Finally, the assay was applied to the analysis of TBBPA in electronic waste samples. The results were agreed well with those of liquid chromatography method, proved that the established immunoassay were accurate and receivable. It is believed that this BA-ELISA method was suitable for rapid and sensitive screening of TBBPA in environmental monitoring.

No fo Author(s) : 2
Page(s) : 326 - 330
Electronic ISSN : A-F
Volume 1 : Issue 1
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