Journals Proceedings

Abstract

Tomato leaf curl virus disease (ToLCVD) is one of the major causes for maximum yield loss of tomato. This disease is caused by tomato leaf curl viruses (ToLCV) belongs to the genus Begomovirus. The infected plants emerge with severe leaf curling, puckering, and yellow margin, reduced leaf size and stunted growth. Tomato leaf curl symptoms caused due to physiological reason sometimes may misguide the farmer for proper identification. In this study, we described about the molecular diagnosis of ToLCVD in the samples collected from six major tomato cultivating areas of Bangladesh. Molecular methods were followed to diagnose and characterize tomato leaf curl virus coat protein (CP) gene. We have isolated total DNA from symptomatic leaves and subjected to rolling circle amplification (RCA). Restriction digestion, polymerase chain reaction (PCR) and sequencing was performed using the RCA products. The coat protein of ToLCV was sequenced by the begomovirus specific “BGCP” forward and reverse primers. The partial sequences of all the six isolates showed more than 90% nucleotide (nt) identities with tomato leaf curl virus coat protein (CP) gene. The technique describe above can be applied for the easy and proper molecular diagnosis of the ToLCVD for the development of sustainable strategies to manage this disease.